Download e-book for iPad: Biomembranes Part F: Bioenergetics: Oxidative by Sidney Fleischer, Lester Packer

By Sidney Fleischer, Lester Packer

ISBN-10: 0121819558

ISBN-13: 9780121819552

The significantly acclaimed laboratory normal, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. on the grounds that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this day - really a vital book for researchers in all fields of lifestyles sciences

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I. Photographs showing typical appearance of bottles after centrifugation. (A) First low-speed sedimentation of the homogenate. The supernatant (S) ("postnuclear supernarant") is carefully decanted from the residue (R) ("nuclear fraction"). Some fat can be seen as a float on the side opposite the residue. (B) Separation of the "nuclear fraction" using a two-layered step gradient. After centrifugation there is a float and a pellet. The pellet contains two distinct layers. The upper band (brown) is enriched in mitochondria (M); the lower, grayish-red band can be used to prepare nuclei (N).

13 A. Blouin, R. P. Bolender, and E. R. Weibel, J. Cell Biol. 72, 441 (1977). 14 F. Leighton, B. Poole, H. Beaufay, P. Baudhuin, J. W. Coffey, S. Fowler, and C. de Duve, J. Cell Biol. 37, 482 (1968). 15 E. Bustamante, J. W. Soper, and P. L. Pederson. A High Yield Preparative Method for Isolation of Rat Liver Mitochondria. Anal. Biochem. 80, 401 (1977). [4] I s o l a t i o n o f M i t o c h o n d r i a w i t h E m p h a s i s o n H e a r t Mitochondria from Small Amounts of Tissue By LEENA MELA and STEVEN SEITZ The fibrous structure of muscle tissue and the contaminating collagenous material around the fibers create particular problems for the isolation of heart mitochondria.

Klingenberg, and T. Bficher, Eur. J. Biochem. 14, 75 (1970). 175G. W. de Goede, J. Samallo, M. Haltrop, and G. L. Scherphof, Biochim. Biophys. Acta 424, 195 (1976). lrrD. L. Edwards, E. Rosenberg, and P. A. Maroney, J. Biol. Chem. 249, 3551 (1974). 177R. Michel, A. Liebl, W. Machleidt, J. Otto, and W. Neupert, Hoppe-Seyler's Z. Physiol. Chem. 356, 1595 (1975). trsS. Werner, Eur. J. Biochem. 43, 39 (1974). 1795. Werner, A. J. Schwab, and W. Neupert, Eur. J. Biochem. 49, 607 (1974). [1 ] MITOCHONDRIAL PREPARATIONS AND PROPERTIES 27 for 30-90 min until protoplasts are formed.

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Biomembranes Part F: Bioenergetics: Oxidative Phosphorylation by Sidney Fleischer, Lester Packer

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