F-5 in Action No 38 - download pdf or read online

By Lou Drendel

ISBN-10: 0897470958

ISBN-13: 9780897470957

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COOPER ET AL. 24 binding. A gradual decline was seen with increasing concentrations of the. agent. However, the effect on the binding in the presence of GTP was profound. Low concentrations of NEM in the pre-treatment (up to 50 VM) resulted in almost a complete loss in the GTP-promoted increase in binding (Fig. 5). That this was not a complete loss, but only a crossover point in passing from a GTP-dependent increase to a GTP-dependent decrease, is seen by examining the effects of higher treatments of NEM.

D~assel and Z. Selinger, Mechanism of adenylate cyclase activation by cholera toxin: Inhibition of GTP hydrolysis at the regulatory site, Proc. Natl. Acad. Sci. USA 74:3307 (1977). 12. M. Svoboda, M. Lambert, and J. Schristophe:-Distinct effects of the C-terminal octapeptide of cholecystokinin and of a cholera toxin pretreatment on the kinetics of rat pancreatic adenylate cyclase activity, Biochim. Biophys. Acta 675:46 (1981). 13. K. Aktories, G. H. Jakobs, Cholera toxin inhibits prostaglandin El but not adrenaline-induced stimulation of GTP hydrolysis in human platelet membranes, FEBS Lett.

J. Lefkowitz, Hormone-sensitive adenylate cyclase: Delineation of a trypsin-sensitive site in the path- 44 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. K. AKTORIES ET AL. way of receptor-mediated inhibition, J. BioI. 257:6287 (1982). H. A. Johnson, and G. Schultz, Activation of human platelet adenylate cyclase by a bovine sperm component, Biochim. Biophys. Acta 756:369 (1983). H. Jakobs, P. Lasch, M. Minuth, K. Aktories, and G. Schultz, Uncoupling of a-adrenoceptor-mediated inhibition of human platelet adenylate cyclase by N-ethylmaleimide, J.

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F-5 in Action No 38 by Lou Drendel


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