Download PDF by Harry Walter: Partitioning in Aqueous Two-Phase System. Theory, Methods,

By Harry Walter

ISBN-10: 0127338608

ISBN-13: 9780127338606

ISBN-10: 0127338616

ISBN-13: 9780127338613

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For a system containing a second uni-univalent salt, with the same cation and pH, we have: n r InKp ^ p ++ ^ = = - R^ T - - llnr P R T (42) where the primed symbols refer to the equivalent quantities in Eq. (39), but for the second salt. Assuming that zp = z p , and subtracting Eq. (39) from Eq. (42), we obtain: K' (Δα° - ΔμΖ') , r¿ zpF An expression for the difference in potentials between two systems with a common cation, taken from Section III,B,l,h below, gives: ^ (Δψ' - Δψ) = l n | f + l n g + (Δμ°+ - Δμ0/) (44) Substituting this into Eq.

By contrast, Tilcock and Fisher (1982) and Arnold et al. (1983) found that the bilayer configuration was maintained. PEG solutions can also cause the aggregation of sonicated liposomes (Tilcock and Fisher, 1982). , 1981a). An early study by Maggio et al. (1976) reported that PEG was able to reduce the surface potential of phospholipid monolayers. The concentrations at which this occurred were low, hence these observations may be of significance for phase partition. , 1979), cause the release of membrane components (McGammon and Fan, 1979), and are markedly hyperosmotic (Fisher and Goodall, 1981).

Cell and Particle Partitioning 1. Thermodynamic Theory of Particle Partition a. Relation to Interfacial Tension and Cell Surface Free Energies. A convenient starting point for the discussion of the theoretical aspects of particle partition is the Boltzmann equation. , Guggenheim, 1959), to the energy ΔΕ necessary to move the particle between the compartments. ) K = n a /n 2 = d/c 2 = exp(-AE/kT) (61) where k is Boltzmann's constant and T is the absolute temperature. The assumption behind the use of kT in Eq.

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Partitioning in Aqueous Two-Phase System. Theory, Methods, Uses, And Applications To Biotechnology by Harry Walter


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