By Timothy P. Karpetsky, Mark S. Boguski, Carl C. Levy (auth.), Donald B. Roodyn (eds.)
This quantity maintains the culture of SUBCELLULAR BIOCHEMISTRY of attempting to holiday down interdisciplinary obstacles within the research of mobile functionality and of bringing the reader's consciousness to much less good studied, yet however worthy, organic structures. we begin with an intensive article through T. P. Karpetsky, M. S. Boguski and C. C. Levy at the constitution, houses and attainable features of polyadenylic acid. except revealing a normal loss of appreciation of many vital points of the chemical houses of poly adenylic acid, the literature additionally exhibits that there's a nice gulf among those that learn the organic position of polyadenylic acid. and those that learn its physicochemi cal homes. the item via Karpetsky and his colleagues is an try to conquer this loss of communique and to offer an built-in view of the topic. The authors cross into the topic in complete aspect and the extra biologically vulnerable reader may perhaps every so often need to reread his nucleic acid actual chemistry notes! even though, the hassle is worth it and the item is a well timed reminder that we can't deal with nucleic acids as mere abstractions, yet that they're advanced natural macromolecules able to both complicated, yet however vital, interactions. the following article is by means of J. Steensgaard and N. P. Hundahl M0ller and bargains with computing device simulation of density gradient centrifugation systems.
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A somewhat analogous situation exists for mitochondrial mRNA. , 1973; Ojala and Attardi, 1974a, b). , 1976), and that this homo polymeric purine sequence can have no transport role within this organelle. That cytoplasmic synthesis of poly(A) occurs in eukaryotic cells is a strong indication that the primary role ofpoly(A) is not transport ofmRNA from nucleus to cytoplasm, but that another role exists for this sequence. Viruses that replicate in the cytoplasm, for example, synthesize polyadenylated mRNA (Kates, 1970; Yogo and Wimmer, 1972; Soria and Huang, 1973).
19) [and more familiarly as poly(A) polymerase] and is characterized as having a requirement for either Mn2+ or Mg2+, for ATP rather than the other nucleoside triphosphates, and for ribonucleoside primers (Edmonds and Winters, 1976). Originally described by Edmonds Polyadenylic Acid 25 and Abrams (1960) in calf thymus, the enzyme appears to be ubiquitous in that it has subsequently been isolated from both the nuclei and cytoplasm of a variety of eUkaryotic tissues, from mitochondria, from bacteria, and from viruses [for a review on poly(A) polymerase, see Edmonds and Winters, 1976].
1977). Kaufmann et al. (1977) recently examined the translation products of poly(A)-containing and poly(A)-lacking mRN A fractions from HeLa cells and detected a considerable degree of homology between the two mRNA populations, although some proteins appear to be coded for exclusively by one population or the other. Additionally, in comparison with an earlier Polyadenylic Acid 17 study by Milcarek et al. (1974), more refined cDNA hybridization experiments have revealed a small but significant homology between poly(A)containing and poly(A)-lacking mRNA sequences.
Subcellular Biochemistry: Volume 6 by Timothy P. Karpetsky, Mark S. Boguski, Carl C. Levy (auth.), Donald B. Roodyn (eds.)